Комплект для прямой поставки с мобильным устройством RT

Описание и отзывы

Характеристики

 

 

 

 

 

 

Description:

 

This kit uses a unique lysis buffer system that can quickly release RNA from cultured cell samples for RT-qPCR reactions, thereby eliminating the time-consuming and laborious RNA purification process. The RNA template can be obtained in just 7 minutes. The 5×Direct RT Mix and 2×Direct qPCR Mix-SYBR reagents provided by the kit can quickly and effectively obtain real-time quantitative PCR results.

 

5×Direct RT Mix and 2×Direct qPCR Mix-SYBR have strong inhibitor tolerance, and the lysate of the samples can be used as the template for RT-qPCR directly. This kit contains the unique RNA high-affinity Foregene reverse transcriptase, and Hot D-Taq DNA polymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer and stabilizer.

 

Specifications:

 

200×20μl Rxns, 1000×20μl Rxns

 

Kit components:

 

Part I	Buffer CL
	Foregene Protease Plus II
	Buffer ST
Part II	DNA Eraser
	5× Direct RT Mix
	2× Direct qPCR Mix-SYBR
	50× ROX Reference Dye
	RNase-Free ddH2O
Instructions

 

Features&advantages:

 

-Simple and effective : with Cell Direct RT technology, RNA samples can be obtained in just 7 minutes.

-The sample demand is small, as low as 10 cells can be tested.

-High throughput: it can quickly detect RNA in cells cultured in 384, 96, 24, 12, 6-well plates.

-DNA Eraser can quickly remove released genomes, greatly reduce the impact on subsequent experimental results.

-Optimized RT and qPCR system makes the two-step RT-PCR reverse transcription more efficient and PCR more specific, and more resistant to RT-qPCR reaction inhibitors.

 

Kit application:

 

-Scope of application: cultured cells.

-RNA released by sample lysis: only applicable to the RT-qPCR template of this kit.

-The kit can be used for the following purposes: gene expression analysis, verification of siRNA-mediated gene silencing effect, drug screening, etc.

 

 

Diagram:

 

 

Storage and Shelf life:

 

Part I of this kit should be stored at 4℃; Part II should be stored at -20℃.

-Foregene Protease Plus II should be stored at 4℃,do not freeze at -20℃.

-Reagent 2×Direct qPCR Mix-SYBR should be stored at -20℃ in the dark; if used frequently, it can also be stored at 4℃ for short-term storage (use up within 10 days).

 

 

    Foregene Co., Ltd is specialtized in the IVD kits and R&D reagents production and services, which is integrated in R&D, production and marketing. Foregene has the world’s leading Direct PCR platform.The main products include various IVD kits, nucleic acid purification reagents,PCR and Direct PCR reagents,Genotyping kits, etc. The technology and product performance indicators used are all leading the world status,widely applied in hospitals, institutions, medical laboratories, etc.

 

  Foregene Co., Ltd. was founded in April 2011 by well-known scholars who returned from studying at Yale University, famous inventors and senior industry professionals.Foregene’s R&D team consists of 40 people(mainly phD and master), has 2500 m² comprehensive building, 600 m² P2 laboratory, and 1200m² GMP production facility.

With Direct PCR technology, there’s no need for cumbersome nucleic acid purification, the sample is directly added to the system and then on the machine.Now all indicators of Foregene’s Direct PCR technology meet the needs of clinical diagnosis.