Набор для изоляции ДНК

Описание и отзывы

Характеристики

 

 

 

 

 

 

Description:

 

The blood sample contains a large number of red blood cells and platelet cells, which makes blood processing more troublesome, and the purity and quantity of blood genomic DNA purified by conventional methods is low. The kit uses Foregene Protease Plus and unique BL1 and BL2 buffer systems, so anticoagulated blood and coagulated blood samples can be completely digested in a short time, thereby minimizing DNA degradation and obtaining the maximum amount of genomic DNA. The fast blood processing system and simple spin column operation greatly simplify the purification of blood genome, and high-quality and high-purity genomic DNA can be obtained within 40 minutes.

The DNA-only silica gel membrane used in the spin column of the kit is Foregene's unique new material, which can efficiently and specifically bind DNA, and can remove RNA, impurity proteins, ions and other organic compounds in cells to the greatest extent. The maximum capacity of the spin column is 80 μg DNA. The obtained DNA can be used in molecular biology experiments, such as restriction digestion, PCR, Southern hybridization and DNA library construction.

 

Specifications:

 

100 Preps,250 Preps

 

Kit components:

 

Buffer BL1 , Buffer BL2, Buffer DC

Buffer PW, Buffer WB1, Buffer EB

Foregene Protease Plus

DNA-Only Column

Instructions

 

 

Features&advantages:

 

-No RNase contamination: The DNA-Only Column provided in the kit makes it possible to remove RNA from genomic DNA without additional RNase during the experiment, thereby preventing the laboratory from being contaminated by exogenous RNase.

-Fast speed: Foregene Protease has higher activity than similar proteases and digests tissue samples faster;

-Simple: the genomic DNA purification operation can be completed in 50 minutes.

-Convenient: The centrifugation is performed at room temperature, 4℃ centrifugation and ethanol precipitation of DNA is not required.

-Safety: no organic reagent is used.

-High quality: The purified genomic DNA has large fragments, no RNA, no RNase, and extremely low ion content, which can meet the requirements of various experiments.

 

Kit application:

 

It is suitable for extraction and purification of genomic DNA from fresh or frozen anticoagulated whole blood.

   

Work Flow: 

 

 

 

Diagram:

 

 

Storage and Shelf life:

 

The kit can be stored for 12 months at room temperature (15-25℃), or 2-8℃ for longer time.

 

 

 

  Foregene Co., Ltd is specialtized in the IVD kits and R&D reagents production and services, which is integrated in R&D, production and marketing. Foregene has the world’s leading Direct PCR platform.The main products include various IVD kits, nucleic acid purification reagents,PCR and Direct PCR reagents,Genotyping kits, etc. The technology and product performance indicators used are all leading the world status,widely applied in hospitals, institutions, medical laboratories, etc.

 

 

   Foregene Co., Ltd. was founded in April 2011 by well-known scholars who returned from studying at Yale University, famous inventors and senior industry professionals.Foregene’s R&D team consists of 40 people(mainly phD and master), has 2500 m² comprehensive building, 600 m² P2 laboratory, and 1200m² GMP production facility.

With Direct PCR technology, there’s no need for cumbersome nucleic acid purification, the sample is directly added to the system and then on the machine.Now all indicators of Foregene’s Direct PCR technology meet the needs of clinical diagnosis.