Высококачественный тест нитрофурантоина быстрый AHD для


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Product Description


HoneyPal  AHD

Rapid Test for the presence of Nitrofurantoin(AHD) in honey.

PRINCIPLE

The HoneyPal AHD is competitive test involving one receptor in one single operation, the nitrofurantoin metabolism AHD in the sample compete for the antibody with the antigen coated on the membrane of the test cassette. Then after a color reaction, when the sample is free of nitrofurantoin(AHD), a colour development occurs at the specific capture lines, indicating the absence of the concerned analytes in the sample. On the contrary, the presence of nitrofurantoin(AHD) in the sample will not cause the coloured signal to appear at the specific capture lines.

APPLICATIONS

The HoneyPal AHD is a rapid test that detect simultaneously the presence of nitrofurantoin(AHD) molecules in a honey sample.

INSTRUMENT NEEDED BUT NOT PROVIDED

Banlance
Homogenizer
Termovap Sample Concentrator
Centrifuge



LIMIT OF DETECTION (LOD)

Honey: 0.5 μg/kg (ppb)



SAMPLE PREPARATION (AOZ, AMOZ, SEM, AHD)
1. Weigh 3.0±0.05g of homogenized honey sample into a 15ml polystyrene centrifuge tube,add 4ml of Solution A(Kit provided) and 200μl of Derivatization Reagent(Kit provided) , shake upside down to mix the honey sample pasty.
2. Incubate the centrifuge tube in the 80℃ water bath for 30min.
3. Take out the centrifuge tube and add 1ml of Solution B(Kit provided), cover the cap, mix thoroughly and cool down to room temperature.
4. Add 5ml of Extraction Solution(Kit provided), seal the cap tightly, and rotate the centrifuge tube upside down for 20 to 25times.
Do NOT vortex in this step.
6. Centrifuge for separation: 5min / 3000g / ambient temperature.
Shake the centrifuge tube up and down for 3 to 4 times and Re-do the centrifuge step in case of the upper layer of clear solution is less than 3ml.
7. Transfer 3ml of upper layer of clear solution into a 10ml polystyrene centrifuge tube, dry with 50-60℃ water bath under nitrogen gas / air flow.
8. Add 400μl of Sample Buffer(Kit provided), vortex for 20s, the prepared sample solution is ready to use.



TEST PROCEDURE

1. Read the instruction attentively, choose a clean and dry place to perform the test, wash and dry your hands before starting.
2. Take the kit out of the fridge and wait until the temperature of the reagents reaches the ambient temperature.
3. Calculate how many honey sample will be tested and mark the sample tubes to identify.
4. Take out as many test cassettes as the honey samples to be tested, and make proper marks to match the honey sample.
Operate the test within 60min after the test cassette is exposed.
5. Pipette 80μl of the prepared sample solution into the cassette orifice.
6. Start the timer to incubate for 10 minutes at room temperature (25℃);
During the incubation, The receptors will detect the presence of the AHD in the sample. The reaction will take 10 minutes.
7. Interpret the result.
File the used test cassettes if necessary.



RESULTS INTERPRETATION

Visual Interpretation

The cassettes are embodied with 2 lines, Control line, Test Line, and which are briefly expressed as “C” and “T”. The test results will be determined by the color darkness of the lines. The following diagram describes the result identification.

NEGATIVE: T Line is darker than the C line, which means that, the honey sample contains fewer nitrofurantoin(AHD) than value given..
POSITIVE: T Line is equal to or weaker than the C line, which means that the honey sample contains the nitrofurantoin(AHD) more than the value given.

REMARKS: Control line is used as a quality indicator, which will always appear regardless of the T line. If Control line does NOT appear, it indicates that the result is invalid. Users please check the kit insert again and re-do the assay with new test cassettes.






STORAGE

2-8℃ in cool dark place, do not freeze. The kit will be valid in 12 months. The lot number and expired date are printed on the package.



PRECAUTIONS

 Do the assay following the instruction, do NOT touch the membrane of the cassette.
 Do NOT used the solutions provided from different batch in one assay.
 The cassette is used for only once; Do NOT use it repeatedly.
 The positive result should be further confirmed.


Specification


item
Rapid Test Kit for AHD in Honey
Model Number
SC014H1
Sample Matrix
honey
Detection Limit
0.5μg/kg (ppb) 
Specification
10 test/kit
Certificate
CE, ISO
Operation time
10min
Operation Temp.
Room Temperature
Result Reading Method
Test Line > Control Line>0, means Negative
Test Line ≤ Control Line  means Positive
Control Line = 0 means Invalid (Redo the test with a new test )
Brand Name
Watar
Instrument May Needed
Banlance 
Homogenizer 
Termovap Sample Concentrator 
Centrifuge


Packing & Delivery




Well packed with Cartoon Box


Company Profile




Shaanxi Watar Biotechnology Co.,Ltd was founded by a group of experienced professionals in food / feed / animal disease diagnostics field in Xi'an(the start point of the"Silk Road"). Watar's main business is to provide ELISA Test Kit and Rapid Test Strip for food / feed safety testing and animal disease diagnostics, many of the products have CE certificates. The hot products like B-lactams & tetracyclines combo test, B-lactams & tetracyclines & sulfanilamides 3in1 test, chloramphenicol rapid test,CDV, CPV, CCV rapid Test now are widely used in many countries.


FAQ


1. Can I get Samples?

Yes, of course, the half kit sample is free of charge, please cover the freight at your side.


2. How do WATAR guarantee quality?

Always 3 times quality Control(QC) Test when preparing the raw material, during production, before stocking;
Always a pre-dlivery conformity test to ensure the quality;
Always online after sales service.

3.What can you buy from us?

Food safety rapid test, Pet disease test, Water test, Milk test, Clinic diagnostics, Lab consumables

4. Why should you buy from us not from other suppliers?

WATAR is more professional, reliable.

6. The payment Terms ?

T/T, Paypal, Western Union.

7. The Delivery

We generally use DHL, Fedex, UPS, TNT, your own express carrier is also welcomed.

8. The Trade terms

FOB / CFR / CIF / EXW

9. The Packing

The ELISA Kit is well packed with ice pack in foam box and catoon box.
The Rapid test Kit is well packed in Catoon box.
The Lab consumablles is packed in Catoon box.



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HoneyPal Test


Ref No.
Product 
Detection Limit
Specification
Sample
Operation Time
SC180H1
Acetamiprid Rapid Test
10ppb
10 Test/kit
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10min
SC176H1
Carbendazol(carbendazim) Rapid Test
5ppb
10 Test/kit
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0.1ppb
10 Test/kit
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SC099H1
Flofenicol Rapid Test
0.3ppb
10 Test/kit
honey, tissue
5 to 10min
SC099H2
Flofenicol Rapid Test
0.1ppb
10 Test/kit
honey, fish
8min
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Fluoroquinolones Rapid Test
20-40ppb
50 Test/kit
honey
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SC044H2
Fluoroquinolones Rapid Test
0.8-5ppb
10 Test/kit
honey
10min
SC006H1
Furaltadone Rapid Test
0.5ppb
10 Test/kit
honey
10min
SC004H1
Furazolidone Rapid Test
0.5ppb
10 Test/kit
honey
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SC038H1
Lincomycin Rapid Test
1ppb
10 Test/kit
honey
13min
SC014H1
Nitrofurantoin Rapid Test
0.5ppb
10 Test/kit
honey
10min
SC010H1
Nitrofurazone Rapid Test
0.5ppb
10 Test/kit
honey
10min
SC050H1
Streptomycin and dihydrostreptomycin Rapid Test
10ppb
10 Test/kit
honey
10min
SC043H1
Sulfonamides Rapid Test
4-10ppb
10 Test/kit
honey
8min
SC040H1
Tetracyclines Rapid Test
10-20ppb
10 Test/kit
honey
10min



Новинки - Розница

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