Набор ДНК HiPure D3121 набор для изоляции тканей и
Набор ДНК HiPure D3121 набор для изоляции тканей и
Набор ДНК HiPure D3121 набор для изоляции тканей и
Набор ДНК HiPure D3121 набор для изоляции тканей и

Набор ДНК HiPure D3121 набор для изоляции тканей и


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Цена:60,00 $ - 75,00 $*
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Характеристики



Overview


Product Name: HiPure Tissue DNA Kit
Product Use Case: Isolation total DNA from Tissue, Culture cells
Packaging Size: 50 Preps/box; 250 Preps/box
Storage: Room temperature
Shelf Life: 18 months


Order Information


Catalog #
Product Name
Packaging Size
List Price (FOB)
D312102
HiPure Tissue DNA Kit
50 preps
75 USD
D312103
HiPure Tissue DNA Kit
250 preps
300 USD


Note: when the quantities of 50 Preps/box are more than 5, we prefer to ship you 250 Preps/box instead.


Published Paper


https://pubmed.ncbi.nlm.nih.gov/27138301/
https://pubmed.ncbi.nlm.nih.gov/25629486/
https://pubmed.ncbi.nlm.nih.gov/27939840/




Introduction


This product provide fast and easy methods for purification of total DNA for reliable PCR and Southern blotting.Total DNA(e.g., genomic, viral, mitochondrial) can be purified from tissue and cultured cells.


Procedure


This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris,pH9.0, 0.5mm EDTA).


Specifications


Main function
Isolation total DNA from Tissue, Culture cells
Downstream Applications
PCR, Southern Blot, viral DNA detection etc
Binding medium
Spin column with Glass fiber membrane
Operation Method
Centrifuge or Vacuum
Sample type
tissue and cultured cells
Sample Volumn
Tissue: less than 25 mg
Cultured cells: less than 5 x 10^6
Column Binding Capacity
100 ug
Column holding Volume
800 ul
Minimum Elution Volume
30 ul
Extraction time
30-60 minutes
Operation
manual
Phenol/Chloroform
no
Ethanol Precipitation
no


Kit Contents





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Новинки - Розница

0.0244 s.