Комплект РНК крови HiPure paxвторая R4168 экстракция из трубки paxigene с использованием Спиннинговых колонн кремниевой
- Категории: Specific Reagents >>>
- Поставщик: Guangzhou,Magen,Biotechnology,Co.,Ltd.
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Описание и отзывы
Характеристики
Overview
Product Name: HiPure Paxigne Blood RNA Kit
Product Use Case: Isolation of RNA from paxigene Blood RNA Tube using silica-membrane spin columns.
Packaging Size:10 Preps/box; 50 Preps/box
Storage：Room temperature
Shelf Life: 18 months
Product Use Case: Isolation of RNA from paxigene Blood RNA Tube using silica-membrane spin columns.
Packaging Size:10 Preps/box; 50 Preps/box
Storage：Room temperature
Shelf Life: 18 months
Order Information
Catalog # | Product Name | Packaging Size | List Price (FOB) |
R416802 | HiPure Paxigne Blood RNA Kit | 10 Preps/box | 59.7 USD |
R416803 | HiPure Paxigne Blood RNA Kit | 50 Preps/box | 223.8 USD |
Note: when the quantities of 10 Preps/box are more than 5, we prefer to ship you 50 Preps/box instead.
Introduction
The Kit provides fast purification of high-quality RNA from paxigene Blood RNA Tube using silica-membrane spin columns. RNA purified using the HiPure System is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
Procedure
The Kit is for the purification of total RNA from 2.5 ml human whole blood collected in a PAXgene Blood RNA Tube. Purification begins with a centrifugation step to pellet nucleic acids in the PAXgene Blood RNA Tube. The pellet is washed and resuspended, and incubated in optimized buffers together with proteinase K. DNA wash The lysate is passed through a DNA Mini column. Ethanol is added to adjust binding conditions, and the lysate is applied to a column. RNA is selectively bound to the silica membrane as contaminants pass through. Remaining contaminants are removed in several efficient wash steps. Between the first and second wash steps, the membrane is treated with DNase I to remove trace amounts of bound DNA. After the wash steps, RNA is eluted in elution buffer and heat-denatured.
Specifications
Main function | Isolation of RNA from paxigene Blood RNA Tube using silica-membrane spin columns. |
Applications | RT-PCR, CDNA synthesis, next-generation sequencing |
Binding medium | Spin column with Glass fiber membrane |
Sample type | Blood in the blood preservation tube |
Operation Method | Centrifuge or Vacuum |
Sample Volume | - |
RNA yield | 5-100ug |
Column Binding Capacity | 100 ug |
Column holding Volume Capacity | 800 µl |
Minimum Elution Volume | 30 ul |
Extraction time | less than 1h |
Operation | manual |
Kit Contents
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Новинки - Розница
