Набор Plasmid DNA Maxiprep высокочистый для очистки ДНК 10 предметов

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Характеристики

Plasmid DNA MaxiPrep Kit

 (High Purity, Silica- membrane, Spin Column)

 

 For research use only

 

Kit Content

Content	N1031
RNase A(10mg/ml)	600 ulx2
Solution I	100 ml
Solution II	100 ml
Solution III	120 ml
Wash Buffer PB	120 ml
Wash Buffer  W	40 ml x 3
Elution Buffer (10 mM Tris-HCl, pH 8.5)	20 ml
Spin Columns	10 each

 

Description

Plasmid Maxiprep Kit is designed for rapid and cost-effective large-scale preparation of high quality plasmid DNA from recombinant E.coli cultures. The kit utilizes an exclusive silica-based membrane technology in the form of a convenient spin column. Each spin column can recover 400-1,000 μg of plasmid DNA from 100 ml overnight bacterial culture. The kit can be successfully used for efficient purification of any size plasmids and cosmids. The actual plasmid yield and optimal culture volume depend on the plasmid copy number and medium used for cultivation.

The method is based on silica-membrane spin column, silica particles bound DNA selectively athigh salt concentration and low pH, while protein and other contaminants are wash to remove. The pure DNA is eluted with TE buffer or water. This method requires few manipulations, and is both faster and easier to perform than other organic-based extraction methods. The purified DNA is suitable for all common molecular biology procedures, including restriction digestion, cloning, sequencing, etc.

 

Applications

Purified DNA is free from contaminants and enzyme inhibitors, and typically has A₂₆₀/A₂₈₀ ratios between 1.7 and 1.9, and is suitable for applications such as:

• Restriction digestion
• PCR
• DNA Sequencing
• in vitro transcription

 

Feature.

• Fast - procedure takes only 40 min.
• High yield : 100 ml suspension yield 400-1,000 μg plasmid DNA of high copy
• High purity - Purified DNA is ready for downstream application such as PCR, restriction digestion.

 

Storage

store RNase A at -20°C, other reagents can be store at room temperature for up to 1 year. Any precipitate forms in the buffers during storage, it should be redissolved by incubating the buffers at 37°C before use. After addition of RNase A, the Resuspension Solution can be used for 6 months when stored at 4°C.

 

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